A Comparative Study on GC-MS Profiles and Effect of Ethanolic Extracts of Stem-Bark and Leaves of Daniellia oliveri on Cerebral Sodium Pump and Thiols Status in Fenton Reaction Treated Rat Organs In-vitro

Daniellia oliveri is one of the most extensively utilized medicinal plants in Nigeria and some West African countries for treatment various ailments. In present study, GC-MS profiles effect ethanolic extracts stem bark leaves on lipid peroxidation, Na+/K+-ATPase activity thiols status H2O2 Fe2+ (fenton reaction) assaulted cerebral hepatic tissue homogenates was investigated. The were extracted with absolute ethanol 72hrs concentrated a rotary evaporator. Wistar rats euthanized brains livers removed, homogenized, centrifuged supernatants used activity, thiol assays presence prooxidants (1mM or 10μM Fe2+).The results revealed that extract both leaf stem-bark inhibited peroxidation induced by H2O2, combination this evident reduction adducts formation rat liver brain possessing more efficacy. Furthermore, Na+/K+-ATPase, while H2O2/Fe2+ caused marked levels protein non-protein tissues homogenates. However, extirpated anomaly increasing as well level tissues. Finally, analysis carried out 21 compounds including 1,2-Benzisothiazol-3-amine, 1H-Indole-2-carboxylic acid, 2-Ethylacridine, Ethyl 2-(2-chloroacetamido)-3,3,3-trifluoro-2-(3-fluoroanilino) propionate trans-1-Butyl-2-methylcyclopropane 10 Methylthio-acetonitrile, 2-Methyl-Z,Z-3,13-octadecadienol, Desulphosinigrin 4H-1,2,4-triazole-3,5-diamine leaves, justifying observed biological activities higher potency may be attributed to bioactive constituents found it. This study therefore justifies usage suggests its consideration management degeneration diseases etiology associated oxidative stress.